Mouse Monoclonal Antibody to UQCRC1
Item Information
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Price
Description
UQCRC1 (Ubiquinol-Cytochrome C Reductase Core Protein 1) is a Protein Coding gene. Diseases associated with UQCRC1 include Leukodystrophy, Hypomyelinating, 4 and Alzheimer Disease. Among its related pathways are Respiratory electron transport, ATP synthesis by chemiosmotic coupling, and heat production by uncoupling proteins. and Cardiac muscle contraction. Gene Ontology (GO) annotations related to this gene include ubiquitin protein ligase binding and ubiquinol-cytochrome-c reductase activity. An important paralog of this gene is PMPCB.
Product Overview
Entrez GenelD
7384
Aliases
QCR1; UQCR1; D3S3191
Clone#
1B1B4
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human UQCRC1 (AA: 60-227) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/50 - 1/200
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Oncotarget. 2016 Dec 27;7(52):86490-86499. 2.Med Sci Monit. 2019 Jul 5;25:4982-4991.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using UQCRC1 mAb against human UQCRC1 (AA: 60-227) recombinant protein. (Expected MW is 44.6 kDa)
Western Blot
Figure 3:Western blot analysis using UQCRC1 mAb against HEK293-6e (1) and UQCRC1 (AA: 60-227)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using UQCRC1 mouse mAb against Hela (1), A431 (2), HepG2 (3), HEK293 (4), PC-3 (5), SH-SY5Y (6), mouse brain (7), and rat brain (8) cell lysate.
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of Hela cells using UQCRC1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis
Figure 6:Flow cytometric analysis of Jurkat cells using UQCRC1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded lung cancer tissues using UQCRC1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 8:Immunohistochemical analysis of paraffin-embedded breast cancer tissues using UQCRC1 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.