ULK3 Primary Antibody
Item Information
Catalog #
Size
Price
Description
Enables protein serine/threonine kinase activity. Involved in several processes, including fibroblast activation; protein autophosphorylation; and regulation of smoothened signaling pathway. Located in cytoplasm.
Product Overview
Entrez GenelD
25989
Clone#
5A5G2
Host / Isotype
Mouse / Mouse IgG1
Species Reactivity
Human, Rat
Immunogen
Purified recombinant fragment of human ULK3 expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/50 - 1/200
FCM (Flow Cytometry)
1/200 - 1/400
References
1.Biochem J. 2021 Jul 30;478(14):2811-2823.
2.Elife. 2015 May 26;4:e06547.
2.Elife. 2015 May 26;4:e06547.
Product Image
Western Blot
Figure 1:Western blot analysis using ULK3 mouse mAb against rat testis (1), PC-3 (2), DU145 (3),HEK293 (4),LNCAP (5)and Hela (6) cell lysate.
Immunohistochemical analysis
Figure 2:Immunofluorescence analysis of Hela cells using ULK3* mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunofluorescence analysis
Figure 3:Flow cytometric analysis of HEK293 cells using ULK3 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 4:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using ULK3 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using ULK3 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.