UCHL1
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.
Product Overview
Entrez GenelD
7345
Aliases
NDGOA; PARK5; PGP95; SPG79; PGP9.5; Uch-L1; HEL-117; PGP 9.5; HEL-S-53
Clone#
1B4H3
Host / Isotype
Mouse / Mouse IgG2b
Species Reactivity
Human, Mouse, Rat
Immunogen
Purified recombinant fragment of human UCHL1 (AA: 1-220) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/50 - 1/200
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Arq Neuropsiquiatr. 2020 Jul;78(7):424-429.
2.Theranostics. 2020 May 15;10(13):6048-6060.
2.Theranostics. 2020 May 15;10(13):6048-6060.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using UCHL1 mAb against human UCHL1 (AA: 1-220) recombinant protein. (Expected MW is 27.5 kDa)
Western Blot
Figure 3:Western blot analysis using UCHL1 mAb against HEK293-6e (1) and UCHL1 (AA: 1-220)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using UCHL1 mouse mAb against mouse brain (1), and rat brain (2) cell lysate.
Immunofluorescence analysis
Figure 5:Flow cytometric analysis of Hek293 cells using UCHL1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using UCHL1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded brain tissues using UCHL1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 8:Immunohistochemical analysis of paraffin-embedded endometrial cancer tissues using UCHL1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 9:Immunofluorescence analysis of Hela cells using UCHL1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
For Research Use Only. Not for use in diagnostic procedures.