UBE2I Primary Antibody
| Item Information | ||
|---|---|---|
| Catalog # | Size/Concentration | Price |
| Specification | |
|---|---|
| Aliases | P18; UBC9; C358B7.1 |
| ProductOrder | U |
| Clone# | 1B10 |
| Entrez GenelD | 7329 |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
| Host | Mouse |
| Isotype | IgG1 |
| Immunogen | Purified recombinant fragment of human UBE2I expressed in E. Coli. |
| MW | 18kDa |
| Shipping Information | This product will ship in a box containing blue ice at a temperature of 4°C. Learn More |
| Species Reactivity | Human, Monkey |
| Application | |
|---|---|
| ELISA | 1/10000 |
| ICC (Immunocytochemistry) | 1/200 - 1/1000 |
| IHC_P(Immunohistochemistry) | 1/200 - 1/1000 |
| FCM (Flow Cytometry) | 1/200 - 1/400 |
| WB (Western Blot) | 1/500 - 1/2000 |
| Sequence | |
|---|---|
| FULL(1-158) | |
Western Blot
Figure 1: Western blot analysis using UBE2I mAb against human UBE2I (AA: 1-158) recombinant protein. (Expected MW is 45.3 kDa)
Western Blot
Figure 2: Western blot analysis using UBE2I mouse mAb against Hela (1), HepG2 (2), and Cos7 (3) cell lysate.
Immunohistochemical analysis
Figure 3: Immunohistochemical analysis of paraffin-embedded prostate tissues using UBE2I mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 4: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using UBE2I mouse mAb with DAB staining.
Immunofluorescence analysis
Figure 5: Immunofluorescence analysis of HepG2 cells using UBE2I mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric
Figure 6: Flow cytometric analysis of HepG2 cells using UBE2I mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);