TWF1 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes twinfilin, an actin monomer-binding protein conserved from yeast to mammals. Studies of the mouse counterpart suggest that this protein may be an actin monomer-binding protein, and its localization to cortical G-actin-rich structures may be regulated by the small GTPase RAC1.
Product Overview
Entrez GenelD
5756
Aliases
A6; PTK9; MGC23788; MGC41876
Clone#
7C11
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human TWF1 (AA: 335-384 ) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/100
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Cancer Epidemiol Biomarkers Prev. 2010 May;19(5):1356-61. 2.Cancer Epidemiol Biomarkers Prev. 2009 May;18(5):1651-8.
Product Image
Western Blot
Figure 1: Western blot analysis using TWF1 mAb against human TWF1 recombinant protein. (Expected MW is 31.1 kDa)
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using TWF1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using TWF1 mouse mAb with DAB staining.
Western Blot
Figure 2: Western blot analysis using TWF1 mAb against HEK293 (1) and TWF1 (AA: 335-384)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of HeLa cells using TWF1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Flow cytometric
Figure 4: Flow cytometric analysis of HeLa cells using TWF1 mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.