TUBB3 Primary Antibody

Item Information

Catalog #

Size

Price

Description

Tubulin, beta 3, also known as TUBB3. Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non exchangeable site on the alpha-chain. Tubulin is a highly conserved protein with a molecular weight of ~50 kD. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. The two main tubulin isoforms, a- and

Product Overview

Entrez GenelD

10381

Aliases

tubulin, beta 3; MC1R; TUBB4

Clone#

2E9

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human TUBB3 expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1. Histopathology. 2007 Jun;50(7):949-52.
2. Neurochem Res. 2007 Aug;32(8):1387-98.
3. Exp Eye Res. 1995 Apr;60(4):385-400.

Product Image

Western Blot

Figure 1: Western blot analysis using TUBB3 mouse mAb against HepG2 (1), A549 (2) and Hela (3) cell lysate.

Immunofluorescence analysis

Figure 2: Immunofluorescence analysis of PANC-1 cells using TUBB3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Flow cytometric