TUBB2A Primary Antibody
Item Information
Catalog #
Size
Price
Description
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
Product Overview
Entrez GenelD
7280
Aliases
TUBB; TUBB2; dJ40E16.7
Clone#
6A10B2
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human TUBB2A (AA: 25-187) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Clin Cancer Res. 2012 Aug 15;18(16):4441-8.
2. Pathol Int. 2012 Apr;62(4):287-90.
2. Pathol Int. 2012 Apr;62(4):287-90.
Product Image
Western Blot
Figure 1: Western blot analysis using TUBB2A mAb against human TUBB2A recombinant protein. (Expected MW is 43.2 kDa)
Western Blot
Figure 2: Western blot analysis using TUBB2A mAb against HEK293 (1) and TUBB2A (AA: 25-187)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using TUBB2A mouse mAb against HeLa (1), A549 (2), HEK293 (3), Jurkat (4) and PC-12 (5) cell lysate.
Flow cytometric
Figure 4: Flow cytometric analysis of Hela cells using TUBB2A mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.