TUBA4A Primary Antibody

Item Information
Catalog # Size/Concentration Price
32012 100ug $341.00
Specification
AliasesALS22; TUBA1; H2-ALPHA
Clone#1C4E12
Entrez GeneID7277
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeMouse IgG2a
ImmunogenPurified recombinant fragment of human TUBA4A (AA: (299-447)) expressed in E. Coli.
MW50kDa
Application
ELISA1/10000
IHC_P (Immunohistochemistry) 1/200-1/1000
FCM (Flow Cytometry)1/200-1/400
WB (Western Blot)1/500 - 1/2000
Sequence
AA: 299-447
Catalog
32012
$341.00
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Images

ELISA

Cell Culture Products
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)

WESTERN BLOT

Cell Culture Products
Figure 2: Western blot analysis using TUBA4A mAb against human TUBA4A (AA: (299-447)) recombinant protein. (Expected MW is 36.9 kDa)

WESTERN BLOT

Cell Culture Products
Figure 3: Western blot analysis using TUBA4A mouse mAb against A431 (1), Hela (2), HepG2 (3), Jurkat (4), Cos7 (5),C6 (6), NIH3T3 (7), HEK293 (8),and HEK293-6e (9) cell lysate.

FLOW CYTOMETRY

Cell Culture Products
Figure 4: Flow cytometric analysis of A431 cells using TUBA4A mouse mAb (green) and negative control (red).

FLOW CYTOMETRY

Cell Culture Products
Figure 5: Flow cytometric analysis of Hepg2 cells using TUBA4A mouse mAb (green) and negative control (red).

IMMUNOHISTOCHEMISTRY

Cell Culture Products
Figure 6: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using TUBA4A mouse mAb with DAB staining.

IMMUNOHISTOCHEMISTRY

Cell Culture Products
Figure 7: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using TUBA4A mouse mAb with DAB staining.
Product Overview
Description

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013]

References (references)
References (references) 1,J Neurol Neurosurg Psychiatry. 2018 Dec;89(12):1350-1352.2,Neurodegener Dis. 2017;17(4-5):171-180.

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