Mouse Monoclonal Antibody to TSPAN8

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Description

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This gene is expressed in different carcinomas. The use of alternate polyadenylation sites has been found for this gene.

Product Overview
Entrez GenelD
7103
Aliases
CO-029; TM4SF3
Clone#
8A4A10
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human TSPAN8 (AA: Extra(110-205)) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,Int J Oncol. 2018 Feb;52(2):473-484. 2,Biomolecules. 2020 Mar 3;10(3):388.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using TSPAN8 mAb against human TSPAN8 (AA: Extra(110-205)) recombinant protein. (Expected MW is 37 kDa)
Figure 2:Western blot analysis using TSPAN8 mAb against human TSPAN8 (AA: Extra(110-205)) recombinant protein. (Expected MW is 37 kDa)
Western Blot
Figure 3:Western blot analysis using TSPAN8 mAb against HEK293-6e (1) and human TSPAN8 (AA: Extra(110-205))-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 3:Western blot analysis using TSPAN8 mAb against HEK293-6e (1) and human TSPAN8 (AA: Extra(110-205))-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using TSPAN8 mouse mAb against HT-29 cell lysate.
Figure 4:Western blot analysis using TSPAN8 mouse mAb against HT-29 cell lysate.
Flow cytometric analysis
Figure 5:Flow cytometric analysis of HepG2 cells using TSPAN8 mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of HepG2 cells using TSPAN8 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded liver cancer tissues using TSPAN8 mouse mAb with DAB staining.
Figure 6:Immunohistochemical analysis of paraffin-embedded liver cancer tissues using TSPAN8 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using TSPAN8 mouse mAb with DAB staining.
Figure 7:Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using TSPAN8 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 8:Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using TSPAN8 mouse mAb with DAB staining.
Figure 8:Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using TSPAN8 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.