Mouse Monoclonal Antibody to TSPAN16

Item Information

Catalog #

Size

Price

Description

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein might couple to signal transduction pathways and possibly modulate cellular activation and adhesion in haemopoietic and neural tissue. Several transcript variants encoding different isoforms have been found for this gene.

Product Overview

Entrez GenelD

26526

Aliases

TM-8; TM4-B; TM4SF16

Clone#

5A2C10

Host / Isotype

Mouse / IgG1

Immunogen

Purified recombinant fragment of human TSPAN16 (AA: 116-245) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1.J Med Genet. 2013 May;50(5):298-308.2.Biochim Biophys Acta. 1999 Oct 6;1447(1):93-9.

Product Image

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using TSPAN16 mAb against human TSPAN16 (AA: 116-245) recombinant protein. (Expected MW is 40.3 kDa)

Western Blot

Figure 3:Western blot analysis using TSPAN16 mAb against HEK293-6e (1) and TSPAN16 (AA: 116-245)-hIgGFc transfected HEK293-6e (2) cell lysate.

Western Blot

Figure 4:Western blot analysis using TSPAN16 mouse mAb against Raji (1), HL-60 (2), Hela (3), A549 (4), THP-1 (5), Mouse liver (6), C6 (7), COS7 (8) and Jurkat (9) cell lysate.

Flow cytometric analysis