Mouse Monoclonal Antibody to TSPAN16

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesTM-8; TM4-B; TM4SF16
Clone#5A2C10
Entrez GenelD26526
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human TSPAN16 (AA: 116-245) expressed in E. Coli.
MW26.2kDa
Application
ELISA1/10000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
116-245
Catalog#: 32251
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Images
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using TSPAN16 mAb against human TSPAN16 (AA: 116-245) recombinant protein. (Expected MW is 40.3 kDa)
Figure 2:Western blot analysis using TSPAN16 mAb against human TSPAN16 (AA: 116-245) recombinant protein. (Expected MW is 40.3 kDa)
Western Blot
Figure 3:Western blot analysis using TSPAN16 mAb against HEK293-6e (1) and TSPAN16 (AA: 116-245)-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 3:Western blot analysis using TSPAN16 mAb against HEK293-6e (1) and TSPAN16 (AA: 116-245)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using TSPAN16 mouse mAb against Raji (1), HL-60 (2), Hela (3), A549 (4), THP-1 (5), Mouse liver (6), C6 (7), COS7 (8) and Jurkat (9) cell lysate.
Figure 4:Western blot analysis using TSPAN16 mouse mAb against Raji (1), HL-60 (2), Hela (3), A549 (4), THP-1 (5), Mouse liver (6), C6 (7), COS7 (8) and Jurkat (9) cell lysate.
Flow cytometric analysis
Figure 5:Flow cytometric analysis of U937 cells using TSPAN16 mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of U937 cells using TSPAN16 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using TSPAN16 mouse mAb with DAB staining.
Figure 6:Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using TSPAN16 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded cerebellum tissues using TSPAN16 mouse mAb with DAB staining.
Figure 7:Immunohistochemical analysis of paraffin-embedded cerebellum tissues using TSPAN16 mouse mAb with DAB staining.
Product Overview
Description

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein might couple to signal transduction pathways and possibly modulate cellular activation and adhesion in haemopoietic and neural tissue. Several transcript variants encoding different isoforms have been found for this gene.

References (references)
References (references)1.J Med Genet. 2013 May;50(5):298-308.2.Biochim Biophys Acta. 1999 Oct 6;1447(1):93-9.