Mouse Monoclonal Antibody to TSG101
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression.
Product Overview
Entrez GenelD
7251
Aliases
TSG10; VPS23
Clone#
5H2A12
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human TSG101 (AA: 167-374) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,J Biol Chem. 2019 Nov 1;294(44):16266-16282. 2,Int J Mol Sci. 2019 Feb 12;20(3):774.
Product Image
Western Blot
Figure 2:Western blot analysis using TSG101 mAb against human TSG101 (AA: 167-374) recombinant protein. (Expected MW is 26.3 kDa)
Western Blot
Figure 3:Western blot analysis using TSG101 mAb against HEK293-6e (1) and human TSG101 (AA: 167-374)-hIgGFc transfected HEK293-6e (2) cell lysate.
Flow cytometric analysis
Figure 3:Flow cytometric analysis of THP-1 cells using TSG101 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.