TRIM29 Primary Antibody

Item Information

Catalog #

Size

Price

Description

The protein encoded by this gene belongs to the TRIM protein family. It has multiple zinc finger motifs and a leucine zipper motif. It has been proposed to form homo- or heterodimers which are involved in nucleic acid binding. Thus, it may act as a transcriptional regulatory factor involved in carcinogenesis and/or differentiation. It may also function in the suppression of radiosensitivity since it is associated with ataxia telangiectasia phenotype.

Product Overview

Entrez GenelD

23650

Aliases

ATDC

Clone#

8C8G5

Host / Isotype

Mouse / IgG2a

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human TRIM29 (AA: 451-588) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/100 - 1/500

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1.Oncotarget. 2016 Mar 22;7(12):13634-50.
2.Dis Markers. 2014;2014:317817.

Product Image

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using TRIM29 mAb against human TRIM29 (AA: 451-588) recombinant protein. (Expected MW is 41.4 kDa)

Western Blot

Figure 3:Western blot analysis using TRIM29 mAb against HEK293 (1) and TRIM29 (AA: 451-588)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot

Figure 4:Western blot analysis using TRIM29 mouse mAb against Hela (1), HepG2 (2), LOVO (3), and A431 (4) cell lysate.

Immunofluorescence analysis

Figure 5:Immunofluorescence analysis of Hela cells using TRIM29 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric