TRIM28 Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.
Product Overview
Entrez GenelD
10155
Aliases
KAP1; TF1B; RNF96; TIF1B
Clone#
3G10A3
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human TRIM28 expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/100
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Cell Host Microbe. 2011 Jun 16;9(6):484-95.
2.J Biol Chem. 2009 Dec 18;284(51):35670-80.
2.J Biol Chem. 2009 Dec 18;284(51):35670-80.
Product Image
Western Blot
Figure 1: Western blot analysis using TRIM28 mouse mAb against HEK293 (1) and HepG2 (2) cell lysate.
Immunofluorescence analysis
Figure 2: Immunofluorescence analysis of HepG2 cells using TRIM28 mouse mAb (green).
Flow cytometric
Figure 3: Immunohistochemical analysis of paraffin-embedded colon cancer tissues using TRIM28 mouse mAb with DAB staining.
Flow cytometric
Figure 4: Flow cytometric analysis of HEK293 cells using TRIM28 mouse mAb (green) and negative control (purple).
Immunohistochemical analysis
Figure 5: Immunohistochemical analysis of paraffin-embedded breast cancer tissues using TRIM28 mouse mAb with DAB staining.
Elisa
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
For Research Use Only. Not for use in diagnostic procedures.