Mouse Monoclonal Antibody to TRIB2
This gene encodes one of three members of the Tribbles family. The Tribbles members share a Trb domain, which is homologous to protein serine-threonine kinases, but lacks the active site lysine and probably lacks a catalytic function. The Tribbles proteins interact and modulate the activity of signal transduction pathways in a number of physiological and pathological processes. This Tribbles member induces apoptosis of cells mainly of the hematopoietic origin. It has been identified as a protein up-regulated by inflammatory stimuli in myeloid (THP-1) cells, and also as an oncogene that inactivates the transcription factor C/EBPalpha (CCAAT/enhancer-binding protein alpha) and causes acute myelogenous leukemia. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2009]
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/fd9b1bd346f2d69acc5be9d5ed916b3eb51f291f12556bd299c3a2e25a67bc8e.jpg?v=1666706022)
![Figure 2:Western blot analysis using TRIB2 mAb against human TRIB2 (AA:1-200) recombinant protein. (Expected MW is 25.7 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/d18042eef86e4d900fc79107d39a3d89fc1aee185f0648c14ed98e67267e019b.jpg?v=1666706022)
![Figure 3:Western blot analysis using TRIB2 mAb against HEK293 (1) and TRIB2 (AA:1-200)-hIgGFc transfected HEK293 (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/ba7234dd0673704ae242cfbf28cf89c87e30dcc310e6d4e5e987495127b99aa1.jpg?v=1666706022)
![Figure 4:Western blot analysis using TRIB2 mouse mAb against Jurkat (1), MCF-7 (2), SW480 (3),and A375 (4) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/de5fa98a30dcf91d23e9867c9babaa2fff8413c09ac4dc274c5e4980b1fc2201.jpg?v=1666706022)
![Figure 5:Immunofluorescence analysis of Hela cells using TRIB2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/7df8aaa987feeb7ba8fd763ba89392d964f414bb8b1030545c07d981967aff51.jpg?v=1666706022)
![Figure 6:Flow cytometric analysis of Jurkat cells using TRIB2 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/59788b5c239880da97c1e6fd1ee3e35bd79f9d4a08a60be34911824f6ab32a66.jpg?v=1666706022)
![Figure 7:Flow cytometric analysis of K562 cells using TRIB2 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/03ba54b35b8871381111361be69b8a2e7bd99f4739755b26099b73a8bafbb43a.jpg?v=1666706022)