Mouse Monoclonal Antibody to TRBC1

Item Information
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Description

TRBC1 (T Cell Receptor Beta Constant 1) is a Protein Coding gene. Among its related pathways are Translocation of ZAP-70 to Immunological synapse and Innate Immune System. An important paralog of this gene is TRBC2.

Product Overview
Entrez GenelD
28639
Aliases
TCRB; TCRBC1; BV05S1J2.2
Clone#
5A8A10
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human TRBC1 (AA: 1-149) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Proc Natl Acad Sci U S A. 1985 Aug;82(15):5068-72. 2.Virchows Arch. 2005 Jan;446(1):15-20.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using TRBC1 mAb against human TRBC1 (AA: 1-149) recombinant protein. (Expected MW is 42.8 kDa)
Figure 2:Western blot analysis using TRBC1 mAb against human TRBC1 (AA: 1-149) recombinant protein. (Expected MW is 42.8 kDa)
Western Blot
Figure 3:Western blot analysis using TRBC1 mAb against HEK293-6e (1) and TRBC1 (AA: 1-149)-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 3:Western blot analysis using TRBC1 mAb against HEK293-6e (1) and TRBC1 (AA: 1-149)-hIgGFc transfected HEK293-6e (2) cell lysate.
Flow cytometric analysis
Figure 4:Flow cytometric analysis of JURKAT cells using TRBC1 mouse mAb (green) and negative control (red).
Figure 4:Flow cytometric analysis of JURKAT cells using TRBC1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded spleen tissues using TRBC1 mouse mAb with DAB staining.
Figure 5:Immunohistochemical analysis of paraffin-embedded spleen tissues using TRBC1 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.