TRAFD1 Primary Antibody
Item Information
Catalog #
Size
Price
Description
The innate immune system confers host defense against viral and microbial infection, and TRAFD1 is a negative feedback regulator that controls excessive immune responses
Product Overview
Entrez GenelD
10906
Aliases
FLN29
Clone#
8E6E7
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human TRAFD1 (AA: 401-582) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/100
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.J Biol Chem. 2008 Dec 5;283(49):33858-64.
2.J Biol Chem. 2005 Dec 16;280(50):41289-97.
2.J Biol Chem. 2005 Dec 16;280(50):41289-97.
Product Image
Western Blot
Figure 1: Western blot analysis using TRAFD1 mAb against human TRAFD1 recombinant protein. (Expected MW is 45 kDa)
Western Blot
Figure 2: Western blot analysis using TRAFD1 mAb against HEK293 (1) and TRAFD1 (AA: 401-582)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using TRAFD1 mouse mAb against HEK293 (1), Raji (2), and Jurkat (3) cell lysate.
Immunofluorescence analysis
Figure 4: Immunofluorescence analysis of HepG2 cells using TRAFD1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Flow cytometric
Figure 5: Flow cytometric analysis of HeLa cells using TRAFD1 mouse mAb (green) and negative control (purple).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.