TPSAB1
Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, alpha and beta 1. Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils, alpha tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders.
2.J Allergy Clin Immunol. 2021 Feb;147(2):622-632.
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/32399ea.jpg?v=1669029734)
![Figure 2:Western blot analysis using TPSAB1 mAb against human TPSAB1 (AA: 31-275) recombinant protein. (Expected MW is 30.3 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/32399rwb.jpg?v=1669029734)
![Figure 3:Western blot analysis using TPSAB1 mAb against HEK293-6e (1) and TPSAB1 (AA: 31-275)-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/32399twb.jpg?v=1669029734)
![Figure 4:Flow cytometric analysis of HepG2 cells using TPSAB1 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/32399fcm.jpg?v=1669029734)
![Figure 5:Immunohistochemical analysis of paraffin-embedded rat kidney tissues using TPSAB1 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/32399ihc.jpg?v=1669029734)
![Figure 6:Immunohistochemical analysis of paraffin-embedded lung cancer tissues using TPSAB1 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/32399ihc-1.jpg?v=1669029734)
![Figure 7:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using TPSAB1 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/32399ihc-2.jpg?v=1669029734)
![Figure 8:Immunohistochemical analysis of paraffin-embedded rabbit kidney tissues using TPSAB1 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/32399ihc-3.jpg?v=1669029734)