TOP2A Primary Antibody

Item Information
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Description

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.

Product Overview
Entrez GenelD
7153
Aliases
TOP2; TP2A
Clone#
6D4B2
Host / Isotype
Mouse / Mouse IgG1
Immunogen
Purified recombinant fragment of human TOP2A (AA: 1100-1530) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4?; -20? for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1.Asian Pac J Cancer Prev. 2018 Dec 25;19(12):3581-3589. 2.BMC Cancer. 2018 Mar 27;18(1):331.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using TOP2A mAb against human TOP2A (AA: 1100-1530) recombinant protein. (Expected MW is 51.2 kDa)
Figure 2:Western blot analysis using TOP2A mAb against human TOP2A (AA: 1100-1530) recombinant protein. (Expected MW is 51.2 kDa)
Western Blot
Figure 3:Western blot analysis using TOP2A mAb against HEK293 (1) and TOP2A (AA: 1100-1530)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using TOP2A mAb against HEK293 (1) and TOP2A (AA: 1100-1530)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using TOP2A mouse mAb against HepG2 (1), Hela (2), Jurkat (3), and K562 (4) cell lysate.
Figure 4:Western blot analysis using TOP2A mouse mAb against HepG2 (1), Hela (2), Jurkat (3), and K562 (4) cell lysate.
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using TOP2A mouse mAb with DAB staining.
Figure 5:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using TOP2A mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.