TOP1
Item Information
Catalog #
Size
Price
Description
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22.
Product Overview
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Image
Western Blot
Figure 1:Western blot analysis using TOP1 mouse mAb against HUVEC (1), Jukat (2),MCF-7 (3),PC-12 (4),Hela (5),NIH/3T3 (6) and HCT116 (7) cell lysate.
Immunohistochemical analysis
Figure 2:Immunofluorescence analysis of Hela cells using TOP1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunofluorescence analysis
Figure 3:Flow cytometric analysis of Hela cells using TOP1 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.