TOP1 Primary Antibody

Item Information

Catalog #

Size

Price

Description

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22.

Product Overview

Entrez GenelD

7150

Aliases

TOPI

Clone#

3D4B1

Host / Isotype

Mouse / Mouse IgG1

Species Reactivity

Human, Mouse

Immunogen

Purified recombinant fragment of human TOP1 expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/100 - 1/400

FCM (Flow Cytometry)

1/200 - 1/400

References

1.Cell. 2021 May 13;184(10):2618-2632.e17.
2.Nat Commun. 2020 Aug 7;11(1):3940.

Product Image

Western Blot

Figure 1:Western blot analysis using TOP1 mouse mAb against HUVEC (1), Jukat (2),MCF-7 (3),PC-12 (4),Hela (5),NIH/3T3 (6) and HCT116 (7) cell lysate.

Immunohistochemical analysis

Figure 2:Immunofluorescence analysis of Hela cells using TOP1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Immunofluorescence analysis