Mouse Monoclonal Antibody to TNNT2
Item Information
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Price
Description
The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined.
Product Overview
Entrez GenelD
7139
Aliases
CMH2; RCM3; TnTC; cTnT; CMD1D; CMPD2; LVNC6
Clone#
4B1D5
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human TNNT2 (AA: 1-295) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Biomarkers. 2020 Dec;25(8):626-633. 2.Biomarkers. 2020 Dec;25(8):613-615.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using TNNT2 mAb against human TNNT2 (AA: 1-295) recombinant protein. (Expected MW is 61.5 kDa)
Western Blot
Figure 3:Western blot analysis using TNNT2 mAb against HEK293-6e (1) and TNNT2 (AA: 1-295)-hIgGFc transfected HEK293-6e (2) cell lysate.
Flow cytometric analysis
Figure 4:Flow cytometric analysis of LNcap cells using TNNT2 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded cardiac muscle tissues using TNNT2 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.