TNNT2
Item Information
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Size
Price
Description
The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined.
Product Overview
Entrez GenelD
7139
Aliases
CMH2; RCM3; TnTC; cTnT; CMD1D; CMPD2; LVNC6
Clone#
4A6E5
Host / Isotype
Mouse / Mouse IgG1
Species Reactivity
Human, Mouse
Immunogen
Purified recombinant fragment of human TNNT2 (AA: full 1-295) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Oncol Rep. 2020 Aug;44(2):628-636.
2.J Am Heart Assoc. 2020 Apr 21;9(8):e015316.
2.J Am Heart Assoc. 2020 Apr 21;9(8):e015316.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using TNNT2 mAb against human TNNT2(AA: full 1-295) recombinant protein. (Expected MW is 61.5 kDa)
Western Blot
Figure 3:Western blot analysis using TNNT2 mAb against HEK293-6e (1) and TNNT2 (AA: full 1-295)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using TNNT2 mouse mAb against mouse heart (1) cell lysate.
Immunofluorescence analysis
Figure 5:Flow cytometric analysis of Hale cells using TNNT2 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded myocardium tissues using TNNT2 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.