Mouse Monoclonal Antibody to TNFSF9
The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This transmembrane cytokine is a bidirectional signal transducer that acts as a ligand for TNFRSF9/4-1BB, which is a costimulatory receptor molecule in T lymphocytes. This cytokine and its receptor are involved in the antigen presentation process and in the generation of cytotoxic T cells. The receptor TNFRSF9/4-1BB is absent from resting T lymphocytes but rapidly expressed upon antigenic stimulation. The ligand encoded by this gene, TNFSF9/4-1BBL, has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine has also been shown to be required for the optimal CD8 responses in CD8 T cells. This cytokine is expressed in carcinoma cell lines, and is thought to be involved in T cell-tumor cell interaction.
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/6e6c4a5cd10d365a0a21266b710270b0cb6c476257fa65dd7ee5cd73b9763ad5.jpg?v=1666705498)
![Figure 2:Western blot analysis using TNFSF9 mAb against human TNFSF9 (AA: Extra(50-254)) recombinant protein. (Expected MW is 24.3kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/7959495472ab950226dddb56d0431335ad9408516adbbb6b2e3a0e58d87ce74e.jpg?v=1666705498)
![Figure 3:Western blot analysis using TNFSF9 mAb against HEK293-6e (1) and TNFSF9 (AA: Extra(50-254)) transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/df9c1bdc85340e682339f770478a0c6438138fd129c67c4ab62f927252e8dcb0.jpg?v=1666705498)
![Figure 5:Flow cytometric analysis of Raji cells using TNFSF9 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/bfb5aca598eda38c751470a504fb2481d5ef962ddc4c276149d0831d478f9ad9.jpg?v=1666705498)
![Figure 6:Flow cytometric analysis of Jurkat cells using TNFSF9 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/42d89aabf3b3931cddfd5b60f54a80d8adc560907d1be2785a138eb461ab5e0e.jpg?v=1666705498)