Mouse Monoclonal Antibody to TNFSF9
The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This transmembrane cytokine is a bidirectional signal transducer that acts as a ligand for TNFRSF9/4-1BB, which is a costimulatory receptor molecule in T lymphocytes. This cytokine and its receptor are involved in the antigen presentation process and in the generation of cytotoxic T cells. The receptor TNFRSF9/4-1BB is absent from resting T lymphocytes but rapidly expressed upon antigenic stimulation. The ligand encoded by this gene, TNFSF9/4-1BBL, has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine has also been shown to be required for the optimal CD8 responses in CD8 T cells. This cytokine is expressed in carcinoma cell lines, and is thought to be involved in T cell-tumor cell interaction.
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/caaacc627ccb61e3b4f3d541379859ce8b6a1d511b6db0be58d9df5043a8f516.jpg?v=1666705503)
![Figure 2:Western blot analysis using TNFSF9 mAb against human TNFSF9 (AA: Extra(50-254)) recombinant protein. (Expected MW is 24.3kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/48d9ccdf7c161a2818a9500f1eb2d2b8419aa188ae8f124e2a6167789c2e8a7a.jpg?v=1666705503)
![Figure 3:Western blot analysis using TNFSF9 mAb against HEK293-6e (1) and TNFSF9 (AA: Extra(50-254)) transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/3a52883fdb7579bf33ba416758402ee775bc4b6c242849fbef3458f804dbe674.jpg?v=1666705503)
![Figure 4:Immunofluorescence analysis of Hela cells using TNFSF9 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/97fd719d5da2d7a185f4644f4377da56e91270327fcc859f7ac65a74a0f29d26.jpg?v=1666705503)
![Figure 5:Flow cytometric analysis of Raji cells using TNFSF9 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/7c0a8a03eb311db32bf4929943c43f205c4fbe8ba98abdee3a121c70ab87e597.jpg?v=1666705503)
![Figure 6:Flow cytometric analysis of Jurkat cells using TNFSF9 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/0973424cf2bf94eb67952193cc11de33bb4c7acbfbf2ca487247bdcdb63a7e71.jpg?v=1666705503)
![Figure 7:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using TNFSF9 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/22adb5ec82699ffcc70698a33e60155374cb24bfc6bfe1452142fab787f0d380.jpg?v=1666705503)
![Figure 8:Immunohistochemical analysis of paraffin-embedded lung cancer tissues using TNFSF9 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/e8adb71686c1fc721d55ebd382fe91a91a03d852eed471533f3c86213d9b1ea3.jpg?v=1666705503)
![Figure 9:Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using TNFSF9 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/8843ab971aae7fb593fadd06ae14a5b48d8cbe123ece8a7c3ec315bcccbf6eaa.jpg?v=1666705503)