Mouse Monoclonal Antibody to TNFSF9

Item Information
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Description

The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This transmembrane cytokine is a bidirectional signal transducer that acts as a ligand for TNFRSF9/4-1BB, which is a costimulatory receptor molecule in T lymphocytes. This cytokine and its receptor are involved in the antigen presentation process and in the generation of cytotoxic T cells. The receptor TNFRSF9/4-1BB is absent from resting T lymphocytes but rapidly expressed upon antigenic stimulation. The ligand encoded by this gene, TNFSF9/4-1BBL, has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine has also been shown to be required for the optimal CD8 responses in CD8 T cells. This cytokine is expressed in carcinoma cell lines, and is thought to be involved in T cell-tumor cell interaction.

Product Overview
Entrez GenelD
8744
Aliases
CD137L; TNLG5A; 4-1BB-L
Clone#
6H10B5
Host / Isotype
Mouse / IgG2b
Immunogen
Purified recombinant fragment of human TNFSF9 (AA: Extra(50-254)) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,Front Immunol. 2019 Oct 2;10:2216. 2,J Dig Dis. 2017 Jul;18(7):395-403.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using TNFSF9 mAb against human TNFSF9 (AA: Extra(50-254)) recombinant protein. (Expected MW is 24.3kDa)
Figure 2:Western blot analysis using TNFSF9 mAb against human TNFSF9 (AA: Extra(50-254)) recombinant protein. (Expected MW is 24.3kDa)
Western Blot
Figure 3:Western blot analysis using TNFSF9 mAb against HEK293-6e (1) and TNFSF9 (AA: Extra(50-254)) transfected HEK293-6e (2) cell lysate.
Figure 3:Western blot analysis using TNFSF9 mAb against HEK293-6e (1) and TNFSF9 (AA: Extra(50-254)) transfected HEK293-6e (2) cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of Hela cells using TNFSF9 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 4:Immunofluorescence analysis of Hela cells using TNFSF9 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis
Figure 5:Flow cytometric analysis of Raji cells using TNFSF9 mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of Raji cells using TNFSF9 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 6:Flow cytometric analysis of Jurkat cells using TNFSF9 mouse mAb (green) and negative control (red).
Figure 6:Flow cytometric analysis of Jurkat cells using TNFSF9 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using TNFSF9 mouse mAb with DAB staining.
Figure 7:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using TNFSF9 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 8:Immunohistochemical analysis of paraffin-embedded lung cancer tissues using TNFSF9 mouse mAb with DAB staining.
Figure 8:Immunohistochemical analysis of paraffin-embedded lung cancer tissues using TNFSF9 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 9:Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using TNFSF9 mouse mAb with DAB staining.
Figure 9:Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using TNFSF9 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.