TNFSF11 Primary Antibody
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Description
This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found.
Product Overview
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
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Elisa

Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot

Figure 2:Western blot analysis using TNFSF11 mAb against human TNFSF11 (AA: 74-308) recombinant protein. (Expected MW is 52.6 kDa)
Western Blot

Figure 3:Western blot analysis using TNFSF11 mAb against HEK293 (1) and TNFSF11 (AA: 74-308)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot

Figure 4:Western blot analysis using TNFSF11 mouse mAb against COS7 (1), Hela (2), U937 (3), HL-60 (4), Raji (5), Ramos (6), Jurkat (7), and SW480 (8) cell lysate.
Immunofluorescence analysis

Figure 5:Immunofluorescence analysis of Hela cells using TNFSF11 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric

Figure 6:Flow cytometric analysis of Hela cells using TNFSF11 mouse mAb (green) and negative control (red).
Immunohistochemical analysis

Figure 7:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using TNFSF11 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.