TNFRSF18 Primary Antibody

Item Information
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Size
Price
Description

This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25(+)CD4(+) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Product Overview
Entrez GenelD
8784
Aliases
AITR; GITR; CD357; GITR-D
Clone#
4H2D6
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human TNFRSF18 (AA: extra 26-162) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Immunol Lett. 2014 Dec;162(2 Pt B):210-6.2.Int J Rheum Dis. 2016 Feb;19(2):199-204.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using TNFRSF18 mAb against human TNFRSF18 (AA: extra 26-162) recombinant protein. (Expected MW is 40.6 kDa)
Figure 2:Western blot analysis using TNFRSF18 mAb against human TNFRSF18 (AA: extra 26-162) recombinant protein. (Expected MW is 40.6 kDa)
Western Blot
Figure 3:Western blot analysis using TNFRSF18 mAb against HEK293 (1) and TNFRSF18 (AA: extra 26-162)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using TNFRSF18 mAb against HEK293 (1) and TNFRSF18 (AA: extra 26-162)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of Jurkat cells using TNFRSF18 mouse mAb (green) and negative control (red).
Figure 4:Flow cytometric analysis of Jurkat cells using TNFRSF18 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using TNFRSF18 mouse mAb with DAB staining.
Figure 5:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using TNFRSF18 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded colon cancer tissues using TNFRSF18 mouse mAb with DAB staining.
Figure 6:Immunohistochemical analysis of paraffin-embedded colon cancer tissues using TNFRSF18 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.