TLT2
| Item Information | ||
|---|---|---|
| Catalog # | Size/Concentration | Price |
| Specification | |
|---|---|
| Aliases | TLT2; TLT-2; C6orf76; dJ238O23.1 |
| Clone# | 4F9D2 |
| Entrez GenelD | 79865 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
| Host | Mouse |
| Isotype | Mouse IgG2a |
| Immunogen | Purified recombinant fragment of human TLT2 (AA: extra 19-268) expressed in E. Coli. |
| MW | 35kDa |
| Species Reactivity | Human |
| Application | |
|---|---|
| ELISA | 1/10000 |
| ICC (Immunocytochemistry) | 1/200 - 1/1000 |
| IHC_P(Immunohistochemistry) | 1/200 - 1/1000 |
| FCM (Flow Cytometry) | 1/200 - 1/400 |
| WB (Western Blot) | 1/500 - 1/2000 |
| Sequence | |
|---|---|
| extra(19-268) | |
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using TLT2 mAb against human TLT2 (AA: 19-268) recombinant protein. (Expected MW is 30 kDa)
Western Blot
Figure 3:Western blot analysis using TLT2 mAb against HEK293-6e (1) and TLT2 (AA: 19-268)-hIgGFc transfected HEK293-6e (2) cell lysate.
Immunohistochemical analysis
Figure 4:Immunofluorescence analysis of Hela cells using TLT2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunofluorescence analysis
Figure 5:Flow cytometric analysis of HEK293 cells using TLT2 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using TLT2 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using TLT2 mouse mAb with DAB staining.