Mouse Monoclonal Antibody to TLR7

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Description

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. The human TLR family comprises 11 members. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. For the recognition of structural components in foreign microorganisms, the various TLRs exhibit different patterns of expression as well; in this way for example, TLR-3, -7, and -8 are essential in the recognition of single-stranded RNA viruses. TLR7 senses single-stranded RNA oligonucleotides containing guanosine- and uridine-rich sequences from RNA viruses, a recognition occuring in the endosomes of plasmacytoid dendritic cells and B cells. This gene is predominantly expressed in lung, placenta, and spleen, and is phylogenetically related and lies in close proximity to another family member, TLR8, on chromosome X.

Product Overview
Entrez GenelD
51284
Aliases
IMD74; TLR7-like
Clone#
3A9E11
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human TLR7 (AA: 420-644) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Microbes Infect. 2020 May-Jun;22(4-5):226-229. 2.Clin Immunol. 2019 Dec;209:108275.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using TLR7 mAb against human TLR7 (AA: 420-644) recombinant protein. (Expected MW is 29 kDa)
Figure 2:Western blot analysis using TLR7 mAb against human TLR7 (AA: 420-644) recombinant protein. (Expected MW is 29 kDa)
Flow cytometric analysis
Figure 3:Flow cytometric analysis of THP-1 cells using TLR7 mouse mAb (green) and negative control (red).
Figure 3:Flow cytometric analysis of THP-1 cells using TLR7 mouse mAb (green) and negative control (red).
Western Blot
Figure 4:Western blot analysis using TLR7 mAb against HEK293-6e (1) and TLR7 (AA: 420-644)-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 4:Western blot analysis using TLR7 mAb against HEK293-6e (1) and TLR7 (AA: 420-644)-hIgGFc transfected HEK293-6e (2) cell lysate.
For Research Use Only. Not for use in diagnostic procedures.

Catalog#: 32263

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