Tlr2 Primary Antibody
Item Information
Catalog #
Size
Price
Description
the activation of toll-like receptor (TLR) 2 induces oxidative stress and inflammation, TLR2 may be directly linked to skeletal muscle atrophy.
Product Overview
Entrez GenelD
24088
Aliases
Ly105
Clone#
7G5F4
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of mouse Tlr2 (AA: 628-777) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1.Biochem Biophys Res Commun. 2015 Apr 10;459(3):534-40.
2.Indian J Exp Biol. 2015 Feb;53(2):82-92.
2.Indian J Exp Biol. 2015 Feb;53(2):82-92.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using Tlr2 mAb against mouse Tlr2 (AA: 628-777) recombinant protein. (Expected MW is 44 kDa)
Western Blot
Figure 3:Western blot analysis using Tlr2 mAb against HEK293 (1) and Tlr2 (AA: 628-777)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of HepG2 cells using Tlr2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
For Research Use Only. Not for use in diagnostic procedures.