Mouse Monoclonal Antibody to TIMD4
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is overexpressed in several tumors and is correlated with enhanced tumor development and metastasis.
Product Overview
Entrez GenelD
91937
Aliases
TIM4; SMUCKLER
Clone#
2A1H5
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human TIMD4 (AA: extra 25-314) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Cancer Lett. 2018 Nov 1;436:119-128. 2.Med Mol Morphol. 2017 Dec;50(4):220-226.
Product Image
Elisa
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/c60ec0391cfd188926b635f6cdf0ef21bd21bba42095d1340c3768494b8eb4c5.jpg?v=1666705569)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
![Figure 2:Western blot analysis using TIMD4 mAb against human TIMD4 (AA: extra 25-314) recombinant protein. (Expected MW is 34.2 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/b3a119bf3e1a9127844289a5ee69378e69f257741a40309943b29fb2394fd368.jpg?v=1666705569)
Figure 2:Western blot analysis using TIMD4 mAb against human TIMD4 (AA: extra 25-314) recombinant protein. (Expected MW is 34.2 kDa)
Western Blot
![Figure 3:Western blot analysis using TIMD4 mAb against HEK293-6e (1) and TIMD4 (AA: extra 25-314)-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/561609a44f5822898232d7c7f3d4402e80b5ee1c6ea14ef8e3412e9c9c688746.jpg?v=1666705569)
Figure 3:Western blot analysis using TIMD4 mAb against HEK293-6e (1) and TIMD4 (AA: extra 25-314)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
![Figure 4:Western blot analysis using TIMD4 mouse mAb against Jurkat (1), HEK293 (2), HL-60 (3), and Hela (4) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/aa75c71521780cb37269978a36106458d7f66f0d3f172ab9831bde63ddb0e2ee.jpg?v=1666705569)
Figure 4:Western blot analysis using TIMD4 mouse mAb against Jurkat (1), HEK293 (2), HL-60 (3), and Hela (4) cell lysate.
Flow cytometric analysis
![Figure 5:Flow cytometric analysis of Jurkat cells using TIMD4 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/8aaad31d3ef76493917fdf33b7b9151012d3ed131dde5aa8fbac8c47119cd1e8.jpg?v=1666705569)
Figure 5:Flow cytometric analysis of Jurkat cells using TIMD4 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.