Mouse Monoclonal Antibody to TIMD4
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is overexpressed in several tumors and is correlated with enhanced tumor development and metastasis.
Product Overview
Entrez GenelD
91937
Aliases
TIM4; SMUCKLER
Clone#
2C6A3
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human TIMD4 (AA: extra 25-314) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/50 - 1/200
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Cancer Lett. 2018 Nov 1;436:119-128. 2.Med Mol Morphol. 2017 Dec;50(4):220-226.
Product Image
Elisa
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/99d6cbb229b799e2c54d3d23a157c79c2ee8f0abe90ed431873e52a98a7e6b3c.jpg?v=1666705561)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
![Figure 2:Western blot analysis using TIMD4 mAb against human TIMD4 (AA: extra 25-314) recombinant protein. (Expected MW is 34.2 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/0781f1c0b38be9b6503aedde11e4c4ac42d64f797ba5552ecd56e60b38fa3ae9.jpg?v=1666705561)
Figure 2:Western blot analysis using TIMD4 mAb against human TIMD4 (AA: extra 25-314) recombinant protein. (Expected MW is 34.2 kDa)
Western Blot
![Figure 3:Western blot analysis using TIMD4 mAb against HEK293-6e (1) and TIMD4 (AA: extra 25-314)-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/35b326391649912695173e75bf39221d927c943079ddfad694bd5aca79a7191a.jpg?v=1666705561)
Figure 3:Western blot analysis using TIMD4 mAb against HEK293-6e (1) and TIMD4 (AA: extra 25-314)-hIgGFc transfected HEK293-6e (2) cell lysate.
Immunofluorescence analysis
![Figure 4:Immunofluorescence analysis of Jurkat cells using TIMD4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/03197b0d02c202fd96262bcdeb50d5aaced0fd3f12a7b0e5c70826fcfbfa8eba.jpg?v=1666705562)
Figure 4:Immunofluorescence analysis of Jurkat cells using TIMD4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis
![Figure 5:Flow cytometric analysis of Hela cells using TIMD4 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/3d596743feacbe54ac7c3e02f567d0229f30582b8b282cbb1ce092b39a358682.jpg?v=1666705562)
Figure 5:Flow cytometric analysis of Hela cells using TIMD4 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.