TIM3 Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene belongs to the immunoglobulin superfamily, and TIM family of proteins. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas, Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. This protein is a Th1-specific cell surface protein that regulates macrophage activation, and inhibits Th1-mediated auto- and alloimmune responses, and promotes immunological tolerance.
Product Overview
Entrez GenelD
84868
Aliases
HAVCR2; CD366; KIM-3; TIMD3; Tim-3; TIMD-3; HAVcr-2
Clone#
2A7A6
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human TIM3 (AA: 224-301) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Tumour Biol. 2016 Jun;37(6):8209-18.2.Int Immunopharmacol. 2015 Dec;29(2):635-641.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using TIM3 mAb against human TIM3 (AA: 224-301) recombinant protein. (Expected MW is 34.9 kDa)
Western Blot
Figure 3:Western blot analysis using TIM3 mAb against HEK293 (1) and TIM3 (AA: 224-301)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of HL-60 cells using TIM3 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.