TIA1 Primary Antibody

Item Information

Catalog #

Size

Price

Description

The product encoded by this gene is a member of a RNA-binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly(A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15-kDa protein that is thought to be derived from the carboxyl terminus of the 40-kDa product by proteolytic processing. Alternative splicing resulting in different isoforms has been found for this gene.

Product Overview

Entrez GenelD

7072

Aliases

WDM; TIA-1

Clone#

3A3B9

Host / Isotype

Mouse / Mouse IgG1

Immunogen

Purified recombinant fragment of human TIA1 (AA: 1-215) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4°C; -20°C for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200-1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200-1/400

ELISA

1/10000

References

1.Cell Rep. 2018 Jan 2;22(1):59-71. 2.Oncotarget. 2016 Mar 29;7(13):17111-28.

Product Image

ELISA

Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)

WESTERN BLOT

Figure 2: Western blot analysis using TIA1 mAb against human TIA1 (AA: 1-215) recombinant protein. (Expected MW is 26.8 kDa)

WESTERN BLOT

Figure 3: Western blot analysis using TIA1 mAb against HEK293 (1) and TIA1 (AA: 1-215)-hIgGFc transfected HEK293 (2) cell lysate.

IMMUNOFLUORESCENCE ANALYSIS

Figure 4: Immunofluorescence analysis of Hela cells using TIA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

FLOW CYTOMETRY