Thioredoxin (TRX) Primary Antibody
Item Information
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Price
Description
Thioredoxin (TRX) is a small ubiquitous protein (MW12kDa) which is exist in a wide variety of prokaryotic and eukaryotic cells. Trx contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. This antibody is suitable for detecting fusion proteins which encode a Trx-Tag by immunoblotting and immunoprecipitation. The Monoclonal Antibody can detect a little Trx-Tag fusion proteins with negligible cross-reactivity with bacterial, insect, or mammalian lysates.
Product Overview
Aliases
Thioredoxin
Clone#
1H6H6
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fusion protein with Thioredoxin (TRX) tag.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ELISA
1/10000
References
1. Holmgen, A. et al., Annu. Rev. Biochem. 54, 237-271 (1985).
2. Wollman, E. E. et al., J. Biol. Chem. 263, 15506-15512 (1988).
3. Sasada, T. et al., J. Toxicol. Sci. 21, 285-287 (1996).
2. Wollman, E. E. et al., J. Biol. Chem. 263, 15506-15512 (1988).
3. Sasada, T. et al., J. Toxicol. Sci. 21, 285-287 (1996).
Product Image
Western Blot
Figure 1: Western blot analysis using Trx mouse mAb against various fusion protein with Trx tag.
For Research Use Only. Not for use in diagnostic procedures.