THAP1 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesDYT6
ProductOrderT
Clone#8D10C8
Entrez GenelD55145
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human THAP1 (AA: 1-213) expressed in E. Coli.
MW24.9kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
full length(1-213)
Catalog#: 30819
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Images
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using THAP1 mAb against human THAP1 (AA: 1-213) recombinant protein. (Expected MW is 50.3 kDa)
Figure 2:Western blot analysis using THAP1 mAb against human THAP1 (AA: 1-213) recombinant protein. (Expected MW is 50.3 kDa)
Western Blot
Figure 3:Western blot analysis using THAP1 mAb against HEK293 (1) and THAP1 (AA: 1-213)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using THAP1 mAb against HEK293 (1) and THAP1 (AA: 1-213)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of Hela cells using THAP1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 4:Immunofluorescence analysis of Hela cells using THAP1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 5:Flow cytometric analysis of Jurkat cells using THAP1 mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of Jurkat cells using THAP1 mouse mAb (green) and negative control (red).
Product Overview
DescriptionThe protein encoded by this gene contains a THAP domain, a conserved DNA-binding domain. This protein colocalizes with the apoptosis response protein PAWR/PAR-4 in promyelocytic leukemia (PML) nuclear bodies, and functions as a proapoptotic factor that links PAWR to PML nuclear bodies. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
References (references)
References (references)1.Mov Disord. 2014 Feb;29(2):278-80.
2.Hum Mutat. 2011 Nov;32(11):1213-24.