TFF2 Primary Antibody

Item Information
Catalog #
Size
Price
Description

Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. The encoded protein inhibits gastric acid secretion. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21.

Product Overview
Entrez GenelD
7032
Aliases
SP; SML1
Clone#
4G7C3
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human TFF2 (AA: 20-125) expressed in E. Coli.
Formulation
Purified antibody from tissue culture in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Peptides. 2013 Aug;46:1-5.
2. Oncol Rep. 2012 Apr;27(4):1207-12.
Product Image
Western Blot
Figure 1: Western blot analysis using TFF2 mAb against human TFF2 (AA: 20-125) recombinant protein. (Expected MW is 37.3 kDa)
Figure 1: Western blot analysis using TFF2 mAb against human TFF2 (AA: 20-125) recombinant protein. (Expected MW is 37.3 kDa)
Western Blot
Figure 2: Western blot analysis using TFF2 mAb against HEK293 (1) and TFF2 (AA: 20-125)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using TFF2 mAb against HEK293 (1) and TFF2 (AA: 20-125)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 3: Flow cytometric analysis of Hela cells using TFF2 mouse mAb (green) and negative control (red).
Figure 3: Flow cytometric analysis of Hela cells using TFF2 mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.