TFAP2A Primary Antibody

Item Information
Catalog # Size/Concentration Price
31169 100μl $341.00
Specification
AliasesAP-2; BOFS; AP2TF; TFAP2; AP-2alpha
Product OrderT
Clone#2H9B5
Entrez GeneID7020
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human TFAP2A (AA: 105-211) expressed in E. Coli.
MW48kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C.  Learn More
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
105-211
Catalog
31169
$341.00
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Images

Elisa

Cell Culture Products
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Cell Culture Products
Figure 2:Western blot analysis using TFAP2A mAb against human TFAP2A (AA: 105-211) recombinant protein. (Expected MW is 37.5 kDa)

Western Blot

Cell Culture Products
Figure 3:Western blot analysis using TFAP2A mAb against HEK293 (1) and TFAP2A (AA: 105-211)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot

Cell Culture Products
Figure 4:Western blot analysis using TFAP2A mouse mAb against Hela (1), PANC-1 (2), HEK293 (3), and MCF-7 (4) cell lysate.

Immunofluorescence analysis

Cell Culture Products
Figure 5:Immunofluorescence analysis of Hela cells using TFAP2A mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Immunofluorescence analysis

Cell Culture Products
Figure 6:Immunofluorescence analysis of Hela cells using TFAP2A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric

Cell Culture Products
Figure 7:Flow cytometric analysis of Hela cells using TFAP2A mouse mAb (green) and negative control (red).
Product Overview
Description The protein encoded by this gene is a transcription factor that binds the consensus sequence 5'-GCCNNNGGC-3'. The encoded protein functions as either a homodimer or as a heterodimer with similar family members. This protein activates the transcription of some genes while inhibiting the transcription of others. Defects in this gene are a cause of branchiooculofacial syndrome (BOFS). Three transcript variants encoding different isoforms have been found for this gene.
References (references)
References (references) 1.Int J Clin Exp Pathol. 2014 Nov 26;7(12):8666-74.
2.Hum Pathol. 2012 Nov;43(11):1866-74.

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