T Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells.
Product Overview
Entrez GenelD
6862
Aliases
TFT
Clone#
1H9A2
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human T (AA: 257-309 ) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Am J Surg Pathol. 2008 Apr;32(4):572-80.
2.J Clin Neurosci. 2011 Jan;18(1):96-9.
2.J Clin Neurosci. 2011 Jan;18(1):96-9.
Product Image
Western Blot
Figure 1: Western blot analysis using T mAb against human T recombinant protein. (Expected MW is 31.2 kDa)
Western Blot
Figure 2: Western blot analysis using T mAb against HEK293 (1) and T (AA: 257-309)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using T mouse mAb against Raji (1), and Jurkat (2) cell lysate.
Flow cytometric
Figure 4: Flow cytometric analysis of HeLa cells using T mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using T mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using T mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.