SRY Primary Antibody
Item Information
Catalog #
Size
Price
Description
This intronless gene encodes a transcription factor that is a member of the high mobility group (HMG)-box family of DNA-binding proteins. This protein is the testis-determining factor (TDF), which initiates male sex determination. Mutations in this gene give rise to XY females with gonadal dysgenesis (Swyer syndrome); translocation of part of the Y chromosome containing this gene to the X chromosome causes XX male syndrome.
Product Overview
Entrez GenelD
6736
Aliases
TDF; TDY; SRXX1; SRXY1
Clone#
1G4
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human SRY (AA:1-204) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.J Biol Chem. 2009 Dec 18;284(51):35670-80. 2.FASEB J. 2009 Nov;23(11):4029-38.
Product Image
Western Blot
Figure 1: Western blot analysis using SRY mAb against human SRY recombinant protein. (Expected MW is 49.4 kDa)
Western Blot
Figure 2: Western blot analysis using SRY mouse mAb against NTERA-2 (1) cell lysate.
Flow cytometric
Figure 3: Flow cytometric analysis of HepG2 cells using SRY mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 4: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using SRY mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 5: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using SRY mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.