SRC Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesASV; SRC1; c-SRC; p60-Src
ProductOrderS
Clone#1F11
Entrez GenelD6714
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human SRC expressed in E. Coli.
MW60kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
1-189
Catalog#: 30290
Inquire
Contact us to order
Images
Western Blot
Figure 1: Western blot analysis using SRC mAb against human SRC (AA: 1-189) recombinant protein. (Expected MW is 47.8 kDa)
Figure 1: Western blot analysis using SRC mAb against human SRC (AA: 1-189) recombinant protein. (Expected MW is 47.8 kDa)
Western Blot
Figure 2: Western blot analysis using SRC mouse mAb against MCF-7 (1), A431 (2), Hela (3), HEK293 (4), NIH/3T3 (5), PC-12 (6) and Cos7 (7) cell lysate.
Figure 2: Western blot analysis using SRC mouse mAb against MCF-7 (1), A431 (2), Hela (3), HEK293 (4), NIH/3T3 (5), PC-12 (6) and Cos7 (7) cell lysate.
Immunohistochemical analysis
Figure 3: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using SRC mouse mAb with DAB staining.
Figure 3: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using SRC mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 4: Immunohistochemical analysis of paraffin-embedded esophageal tissues using SRC mouse mAb with DAB staining.
Figure 4: Immunohistochemical analysis of paraffin-embedded esophageal tissues using SRC mouse mAb with DAB staining.
Flow cytometric
Figure 5: Flow cytometric analysis of MCF-7 cells using SRC mouse mAb (green) and negative control (red).
Figure 5: Flow cytometric analysis of MCF-7 cells using SRC mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
Description

This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.

References (references)
References (references)1. Clin Cancer Res. 2009 Nov 15;15(22):6852-61.
2. Cell. 2009 Nov 13;139(4):693-706.