SRC Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
Product Overview
Entrez GenelD
6714
Aliases
ASV; SRC1; c-SRC; p60-Src
Clone#
1F11
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human SRC expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Clin Cancer Res. 2009 Nov 15;15(22):6852-61.
2. Cell. 2009 Nov 13;139(4):693-706.
2. Cell. 2009 Nov 13;139(4):693-706.
Product Image
Western Blot
Figure 1: Western blot analysis using SRC mAb against human SRC (AA: 1-189) recombinant protein. (Expected MW is 47.8 kDa)
Western Blot
Figure 2: Western blot analysis using SRC mouse mAb against MCF-7 (1), A431 (2), Hela (3), HEK293 (4), NIH/3T3 (5), PC-12 (6) and Cos7 (7) cell lysate.
Immunohistochemical analysis
Figure 3: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using SRC mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 4: Immunohistochemical analysis of paraffin-embedded esophageal tissues using SRC mouse mAb with DAB staining.
Flow cytometric
Figure 5: Flow cytometric analysis of MCF-7 cells using SRC mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.