SQSTM1 Primary Antibody
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Description
This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.
Product Overview
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Image
Western Blot
Figure 1: Western blot analysis using SQSTM1 mAb against human SQSTM1 recombinant protein. (Expected MW is 39.1 kDa)
Western Blot
Figure 2: Western blot analysis using SQSTM1 mAb against HEK293 (1) and SQSTM1 (AA: 232-356)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using SQSTM1 mouse mAb against Hela (1), Jurkat (2), THP-1 (3), HEK293 (4), A549 (5), MCF-7 (6), HepG2 (7), COS7 (8) and SK-BR-3 (9) cell lysate.
Flow cytometric
Figure 4: Flow cytometric analysis of HEK293 cells using SQSTM1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using SQSTM1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using SQSTM1 mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.