SQSTM1 Primary Antibody

Item Information

Catalog #

Size

Price

Description

This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.

Product Overview

Entrez GenelD

8878

Aliases

p60; p62; A170; OSIL; PDB3; ZIP3; p62B

Clone#

5H7E2

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human SQSTM1 (AA: 232-356) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1.Cancer Sci. 2012 Apr;103(4):760-6.
2.Mol Cell. 2011 Oct 7;44(1):134-46.

Product Image

Western Blot

Figure 1: Western blot analysis using SQSTM1 mAb against human SQSTM1 recombinant protein. (Expected MW is 39.1 kDa)

Western Blot

Figure 2: Western blot analysis using SQSTM1 mAb against HEK293 (1) and SQSTM1 (AA: 232-356)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot

Figure 3: Western blot analysis using SQSTM1 mouse mAb against Hela (1), Jurkat (2), THP-1 (3), HEK293 (4), A549 (5), MCF-7 (6), HepG2 (7), COS7 (8) and SK-BR-3 (9) cell lysate.

Flow cytometric