SPIB Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesSPI-B
ProductOrderS
Clone#4G5
Entrez GenelD6689
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human SPIB (AA: 200-252) expressed in E. Coli.
MW28.8kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman, Mouse
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
200-252
Catalog#: 30404
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Images
Western Blot
Figure 1: Western blot analysis using SPIB mAb against human SPIB recombinant protein. (Expected MW is 32 kDa)
Figure 1: Western blot analysis using SPIB mAb against human SPIB recombinant protein. (Expected MW is 32 kDa)
Western Blot
Figure 2: Western blot analysis using SPIB mouse mAb against A549 (1), PC-3 (2), and NIH3T3 (3) cell lysate.
Figure 2: Western blot analysis using SPIB mouse mAb against A549 (1), PC-3 (2), and NIH3T3 (3) cell lysate.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of HeLa cells using SPIB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 3: Immunofluorescence analysis of HeLa cells using SPIB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric
Figure 4: Flow cytometric analysis of NIH3T3 cells using SPIB mouse mAb (green) and negative control (red).
Figure 4: Flow cytometric analysis of NIH3T3 cells using SPIB mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using SPIB mouse mAb with DAB staining.
Figure 5: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using SPIB mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6: Immunohistochemical analysis of paraffin-embedded esophageal cancerwith DAB staining.
Figure 6: Immunohistochemical analysis of paraffin-embedded esophageal cancerwith DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
Description

The protein encoded by this gene is a transcriptional activator that binds to the PU-box (5'-GAGGAA-3') and acts as a lymphoid-specific enhancer. Four transcript variants encoding different isoforms have been found for this gene.

References (references)
References (references)1.J Gen Virol. 2010 Dec;91(Pt 12):3042-52. 2.Eur J Immunol. 2008 Sep;38(9):2389-400.