SPIB Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is a transcriptional activator that binds to the PU-box (5'-GAGGAA-3') and acts as a lymphoid-specific enhancer. Four transcript variants encoding different isoforms have been found for this gene.
Product Overview
Entrez GenelD
6689
Aliases
SPI-B
Clone#
4G5
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Mouse
Immunogen
Purified recombinant fragment of human SPIB (AA: 200-252) expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.J Gen Virol. 2010 Dec;91(Pt 12):3042-52. 2.Eur J Immunol. 2008 Sep;38(9):2389-400.
Product Image
Western Blot
Figure 1: Western blot analysis using SPIB mAb against human SPIB recombinant protein. (Expected MW is 32 kDa)
Western Blot
Figure 2: Western blot analysis using SPIB mouse mAb against A549 (1), PC-3 (2), and NIH3T3 (3) cell lysate.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of HeLa cells using SPIB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric
Figure 4: Flow cytometric analysis of NIH3T3 cells using SPIB mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using SPIB mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6: Immunohistochemical analysis of paraffin-embedded esophageal cancerwith DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.