SPI1 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesOF; PU.1; SFPI1; SPI-1; SPI-A
ProductOrderS
Clone#5C7
Entrez GenelD6688
FormulationAscitic fluid containing 0.03% sodium azide.


HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human SPI1 (AA: 124-271) expressed in E. Coli.

MW42kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman
Application
ELISA1/10000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
124-271
Catalog#: 30389
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510-740-3625
info@promab.com
Images
Western Blot
Figure 1: Western blot analysis using SPI1 mAb against human SPI1 recombinant protein. (Expected MW is 46.9 kDa)
Figure 1: Western blot analysis using SPI1 mAb against human SPI1 recombinant protein. (Expected MW is 46.9 kDa)
Western Blot
Figure 2: Western blot analysis using SPI1 mAb against HEK293 (1) and SPI1 (AA: 124-271)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 2: Western blot analysis using SPI1 mAb against HEK293 (1) and SPI1 (AA: 124-271)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 3: Flow cytometric analysis of HeLa cells using SPI1 mouse mAb (green) and negative control (red).
Figure 3: Flow cytometric analysis of HeLa cells using SPI1 mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Product Overview
Description

This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene.

References (references)
References (references)1.J Biol Chem. 2011 Jan 21;286(3):1675-82.
2.Leuk Res. 2010 Dec;34(12):1636-46.
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