SPI1 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene.
Product Overview
Entrez GenelD
6688
Aliases
OF; PU.1; SFPI1; SPI-1; SPI-A
Clone#
5C7
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human SPI1 (AA: 124-271) expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.J Biol Chem. 2011 Jan 21;286(3):1675-82.
2.Leuk Res. 2010 Dec;34(12):1636-46.
2.Leuk Res. 2010 Dec;34(12):1636-46.
Product Image
Western Blot
Figure 1: Western blot analysis using SPI1 mAb against human SPI1 recombinant protein. (Expected MW is 46.9 kDa)
Western Blot
Figure 2: Western blot analysis using SPI1 mAb against HEK293 (1) and SPI1 (AA: 124-271)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 3: Flow cytometric analysis of HeLa cells using SPI1 mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.