SOX9 Primary Antibody

Item Information

Catalog #

Size

Price

Description

The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal.

Product Overview

Entrez GenelD

6662

Aliases

CMD1; SRA1; CMPD1

Clone#

1B11

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human SOX9 expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4°C; -20°C for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/200 - 1/1000

ELISA

1/10000

References

1. J Cell Sci. 2009 Jul 1;122(Pt 13):2191-6.
2. J Med Genet. 2009 Oct;46(10):649-56.

Product Image

Western Blot

Figure 1: Western blot analysis using SOX9 mAb against human SOX9 (AA: 147-306) recombinant protein. (Expected MW is 56 kDa)

Western Blot

Figure 2: Western blot analysis using SOX9 mouse mAb against Lovo (1) and SW620 (2) cell lysate.

Immunofluorescence analysis

Figure 3: Immunofluorescence analysis of Hela cells using SOX9 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Elisa