SOX9 Primary Antibody

Item Information
Catalog #
Size
Price
Description

The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal.

Product Overview
Entrez GenelD
6662
Aliases
CMD1; SRA1; CMPD1
Clone#
1B11
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human SOX9 expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1. J Cell Sci. 2009 Jul 1;122(Pt 13):2191-6.
2. J Med Genet. 2009 Oct;46(10):649-56.
Product Image
Western Blot
Figure 1: Western blot analysis using SOX9 mAb against human SOX9 (AA: 147-306) recombinant protein. (Expected MW is 56 kDa)
Figure 1: Western blot analysis using SOX9 mAb against human SOX9 (AA: 147-306) recombinant protein. (Expected MW is 56 kDa)
Western Blot
Figure 2: Western blot analysis using SOX9 mouse mAb against Lovo (1) and SW620 (2) cell lysate.
Figure 2: Western blot analysis using SOX9 mouse mAb against Lovo (1) and SW620 (2) cell lysate.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of Hela cells using SOX9 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Figure 3: Immunofluorescence analysis of Hela cells using SOX9 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.