SOD1 Primary Antibody

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesALS; SOD; ALS1; IPOA; homodimer
ProductOrderS
Clone#6F5
Entrez GenelD6647
FormulationAscitic fluid containing 0.03% sodium azide.
HostMouse
IsotypeIgG1
ImmunogenPurified recombinant fragment of human SOD1 expressed in E. Coli.
MW18kDa
Shipping InformationThis product will ship in a box containing blue ice at a temperature of 4°C. Learn More
Species ReactivityHuman, Mouse
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
full
Catalog#: 20349
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Images
Western Blot
Figure 1: Western blot analysis using SOD1 mouse mAb against Hela (1), NIH/3T3 (2), A549 (3) and A431 (4) cell lysate.
Figure 1: Western blot analysis using SOD1 mouse mAb against Hela (1), NIH/3T3 (2), A549 (3) and A431 (4) cell lysate.
Immunofluorescence analysis
Figure 2: Confocal Immunofluorescence analysis of PANC-1 (left) and SKBR-3 (right) cells using SOD1 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Figure 2: Confocal Immunofluorescence analysis of PANC-1 (left) and SKBR-3 (right) cells using SOD1 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Immunofluorescence analysis
Figure 3: Confocal Immunofluorescence analysis of 3T3-L1 cells using SOD1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Figure 3: Confocal Immunofluorescence analysis of 3T3-L1 cells using SOD1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Flow cytometric
Figure 4: Flow cytometric analysis of A431 cells using SOD1 mouse mAb (green) and negative control (purple).
Figure 4: Flow cytometric analysis of A431 cells using SOD1 mouse mAb (green) and negative control (purple).
Product Overview
Description

SOD1 (superoxide dismutase 1, soluble), also known as ALS. The protein binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis (ALS), a progressive degenerative disease of motor neurons. Rare transcript variants have been reported for this gene.

References (references)
References (references)1. Apoptosis. 2005 May;10(3):499-502.
2. Hum Mol Genet. 2008 Nov 1;17(21):3303-17.