SOD1 Primary Antibody
Item Information
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Size
Price
Description
SOD1 (superoxide dismutase 1, soluble), also known as ALS. The protein binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis (ALS), a progressive degenerative disease of motor neurons. Rare transcript variants have been reported for this gene.
Product Overview
Entrez GenelD
6647
Aliases
ALS; SOD; ALS1; IPOA; homodimer
Clone#
6F5
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Mouse
Immunogen
Purified recombinant fragment of human SOD1 expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Apoptosis. 2005 May;10(3):499-502.
2. Hum Mol Genet. 2008 Nov 1;17(21):3303-17.
2. Hum Mol Genet. 2008 Nov 1;17(21):3303-17.
Product Image
Western Blot
Figure 1: Western blot analysis using SOD1 mouse mAb against Hela (1), NIH/3T3 (2), A549 (3) and A431 (4) cell lysate.
Immunofluorescence analysis
Figure 2: Confocal Immunofluorescence analysis of PANC-1 (left) and SKBR-3 (right) cells using SOD1 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Immunofluorescence analysis
Figure 3: Confocal Immunofluorescence analysis of 3T3-L1 cells using SOD1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Flow cytometric
Figure 4: Flow cytometric analysis of A431 cells using SOD1 mouse mAb (green) and negative control (purple).
For Research Use Only. Not for use in diagnostic procedures.