SOCS3 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene is induced by various cytokines, including IL6, IL10, and interferon (IFN)-gamma. The protein encoded by this gene can bind to JAK2 kinase, and inhibit the activity of JAK2 kinase. Studies of the mouse counterpart of this gene suggested the roles of this gene in the negative regulation of fetal liver hematopoiesis, and placental development.
Product Overview
Entrez GenelD
9021
Aliases
CIS3; SSI3; ATOD4; Cish3; SSI-3; SOCS-3
Clone#
5E12E7F1
Host / Isotype
Mouse / Mouse IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human SOCS3 (AA: 1-225) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1，Genet Test Mol Biomarkers. 2020 Jul;24(7):443-450.
2，Eur J Gastroenterol Hepatol. 2020 Apr;32(4):540-541.
2，Eur J Gastroenterol Hepatol. 2020 Apr;32(4):540-541.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using SOCS3 mAb against human SOCS3 (AA: 1-225) recombinant protein. (Expected MW is 27.7 kDa)
Western Blot
Figure 3:Western blot analysis using SOCS3 mAb against HEK293-6e (1) and SOCS3 (AA:full 1-225)-hIgGFc transfected HEK293-6e (2) cell lysate.
Immunohistochemical analysis
Figure 4:Immunofluorescence analysis of Hela cells using SOCS3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunofluorescence analysis
Figure 5:Flow cytometric analysis of Jurkat cells using SOCS3 mouse mAb (green) and negative control (red).
Immunofluorescence analysis
Figure 6:Flow cytometric analysis of Hela cells using SOCS3 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using SOCS3 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 8:Immunohistochemical analysis of paraffin-embedded muscle tissues using SOCS3 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.