SOCS3

Item Information
Catalog #Size/ConcentrationPrice
Specification
AliasesCIS3; SSI3; ATOD4; Cish3; SSI-3; SOCS-3
Clone#3A10A12
Entrez GenelD9021
FormulationPurified antibody in PBS with 0.05% sodium azide
HostMouse
IsotypeMouse IgG1
ImmunogenPurified recombinant fragment of human SOCS3 (AA: full(1-225)) expressed in E. Coli.
MW24.8KDa
Species ReactivityHuman
Application
ELISA1/10000
ICC (Immunocytochemistry)1/200 - 1/1000
IHC_P(Immunohistochemistry)1/200 - 1/1000
FCM (Flow Cytometry)1/200 - 1/400
WB (Western Blot)1/500 - 1/2000
Sequence
full(1-225)
Catalog#: 32281
Inquire
Contact us to order
Images
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using SOCS3 mAb against human SOCS3 (AA: full(1-225)) recombinant protein. (Expected MW is 27.7 kDa)
Figure 2:Western blot analysis using SOCS3 mAb against human SOCS3 (AA: full(1-225)) recombinant protein. (Expected MW is 27.7 kDa)
Western Blot
Figure 3:Western blot analysis using SOCS3 mAb against HEK293-6e (1) and SOCS3 (AA:full(1-225))-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 3:Western blot analysis using SOCS3 mAb against HEK293-6e (1) and SOCS3 (AA:full(1-225))-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using SOCS3 mouse mAb against Hela cell lysate.
Figure 4:Western blot analysis using SOCS3 mouse mAb against Hela cell lysate.
Immunohistochemical analysis
Figure 5:Immunofluorescence analysis of Hela cells using SOCS3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 5:Immunofluorescence analysis of Hela cells using SOCS3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using SOCS3 mouse mAb with DAB staining.
Figure 7:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using SOCS3 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 8:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using SOCS3 mouse mAb with DAB staining.
Figure 8:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using SOCS3 mouse mAb with DAB staining.
Product Overview
Description

This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene is induced by various cytokines, including IL6, IL10, and interferon (IFN)-gamma. The protein encoded by this gene can bind to JAK2 kinase, and inhibit the activity of JAK2 kinase. Studies of the mouse counterpart of this gene suggested the roles of this gene in the negative regulation of fetal liver hematopoiesis, and placental development.

References (references)
References (references)1,Genet Test Mol Biomarkers. 2020 Jul;24(7):443-450.
2,Eur J Gastroenterol Hepatol. 2020 Apr;32(4):540-541.