SNAI2 Primary Antibody

Item Information

Catalog #

Size

Price

Description

This gene encodes a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporatic cases of neural tube defects.

Product Overview

Entrez GenelD

6591

Aliases

SLUG; WS2D; SLUGH1; SNAIL2

Clone#

4B6D5

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human SNAI2 (AA: 100-200) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1. Cancer Med. 2013 Apr;2(2):144-54.
2. FEBS J. 2012 Aug;279(16):2929-39.

Product Image

Western Blot

Figure 1: Western blot analysis using SNAI2 mAb against human SNAI2 (AA: 100-200) recombinant protein. (Expected MW is 39.8 kDa)

Western Blot

Figure 2: Western blot analysis using SNAI2 mAb against HEK293 (1) and SNAI2 (AA: 100-200)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot

Figure 3: Western blot analysis using SNAI2 mouse mAb against MCF-7 cell lysate.

Immunofluorescence analysis

Figure 4:Immunofluorescence analysis of Hela cells using SNAI2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric