SMAD5 Primary Antibody

Item Information

Catalog #

Size

Price

Description

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). SMAD5 is required for normal development of the cardiovascular system in vivo; lack of the SMAD5 gene results in apoptosis of cardiac myocytes. 3 Upregulation of SMAD5 has been reported to mediate apoptosis of gastric epithelial cells induced by Helicobacter pylori infection. Tissue specificity: Ubiquitous.

Product Overview

Entrez GenelD

4090

Aliases

Dwfc; JV5-1; MADH5; DKFZp781C1895; DKFZp781O1323; SMAD5

Clone#

3H9

Host / Isotype

Mouse / IgG1

Species Reactivity

Human, Rat

Immunogen

Purified recombinant fragment of human SMAD5 expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1. Proc Natl Acad Sci U S A. 2008 Mar 11;105(10):3927-32.
2. Nat Cell Biol. 2008 May;10(5):567-74.

Product Image

Western Blot

Figure 1: Western blot analysis using SMAD5 mouse mAb against Hela (1), SK-N-SH (2), PC-12 (3), Jurkat (4), and K562 (5) cell lysate.

Immunohistochemical analysis

Figure 2: Immunohistochemical analysis of paraffin-embedded brain tissues (left) and lung cancer tissues (right) using SMAD5 mouse mAb with DAB staining.

Immunofluorescence analysis

Figure 3: Immunofluorescence analysis of NTERA-2 cells using SMAD5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Flow cytometric