SMAD4 Primary Antibody
Item Information
Catalog #
Size
Price
Description
Common mediator of signal transduction by TGF-beta (transforming growth factor) superfamily; SMAD4 is the common SMAD (co-SMAD). Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. May act as a tumor suppressor?Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome.
Product Overview
Entrez GenelD
4089
Aliases
JIP; DPC4; MADH4; SMAD4
Clone#
4G1C6
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Mouse
Immunogen
Purified recombinant fragment of human SMAD4 expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. Am J Hum Genet. 2009 Nov;85(5):628-42.
2. Mol Endocrinol. 2010 Mar;24(3):540-51.
2. Mol Endocrinol. 2010 Mar;24(3):540-51.
Product Image
Western Blot
Figure 1: Western blot analysis using SMAD4 mouse mAb against A431 (1), SK-N-SH (2), K562 (3), HepG2 (4) and HUVE12 (5) cell lysate.
Immunofluorescence analysis
Figure 2: Immunofluorescence analysis of NIH/3T3 cells using SMAD4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemical analysis
Figure 3: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using SMAD4 mouse mAb with DAB staining.
Flow cytometric
Figure 4: Flow cytometric analysis of K562 cells using SMAD4 mouse mAb (green) and negative control (purple).
For Research Use Only. Not for use in diagnostic procedures.