SMAD3 Primary Antibody
Item Information
Catalog #
Size
Price
Description
SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.
Product Overview
Entrez GenelD
4088
Aliases
MADH3; JV15-2; HSPC193; HsT17436; MGC60396; DKFZp586N0721; DKFZp686J10186; SMAD3
Clone#
5G11
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Mouse
Immunogen
Purified recombinant fragment of human SMAD3 expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1. PLoS One. 2009 Sep 21;4(9):e7091.
2. Heart Rhythm. 2009 Dec;6(12):1745-50.
3. Am J Pathol. 2010 Mar;176(3):1139-47.
2. Heart Rhythm. 2009 Dec;6(12):1745-50.
3. Am J Pathol. 2010 Mar;176(3):1139-47.
Product Image
Western Blot
Figure 1: Western blot analysis using SMAD3 mouse mAb against A549 (1), Hela (2), Jurkat (3), PC-2 (4) and NIH/3T3 (5) cell lysate.
Immunofluorescence analysis
Figure 2: Immunofluorescence analysis of NIH/3T3 cells using SMAD3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
For Research Use Only. Not for use in diagnostic procedures.