SMAD3 Primary Antibody

Item Information

Catalog #

Size

Price

Description

SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.

Product Overview

Entrez GenelD

4088

Aliases

MADH3; JV15-2; HSPC193; HsT17436; MGC60396; DKFZp586N0721; DKFZp686J10186; SMAD3

Clone#

5G11

Host / Isotype

Mouse / IgG1

Species Reactivity

Human, Mouse

Immunogen

Purified recombinant fragment of human SMAD3 expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/200 - 1/1000

ELISA

1/10000

References

1. PLoS One. 2009 Sep 21;4(9):e7091.
2. Heart Rhythm. 2009 Dec;6(12):1745-50.
3. Am J Pathol. 2010 Mar;176(3):1139-47.

Product Image

Western Blot

Figure 1: Western blot analysis using SMAD3 mouse mAb against A549 (1), Hela (2), Jurkat (3), PC-2 (4) and NIH/3T3 (5) cell lysate.

Immunofluorescence analysis

Figure 2: Immunofluorescence analysis of NIH/3T3 cells using SMAD3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

For Research Use Only. Not for use in diagnostic procedures.